3AGE
Crystal structure of Mglu in its L-glutamate binding form in the presence of 4.3M NaCl
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2009-06-05 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 118.835, 130.933, 73.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.470 - 2.600 |
| Rwork | 0.190 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3if5 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.100 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.093 | 0.280 |
| Number of reflections | 35923 | |
| <I/σ(I)> | 18.1 | 6.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch method | 7.5 | 293 | 10mg/ml protein, 50mM HEPES, 1M ammonium formate, 4.3M NaCl, 0.1M L-glutamate, pH 7.5, Microbatch method, temperature 293K |
