3AEK

Structure of the light-independent protochlorophyllide reductase catalyzing a key reduction for greening in the dark

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-16
Detector	ADSC QUANTUM 210
Spacegroup name	P 1 21 1
Unit cell lengths	81.596, 81.219, 175.928
Unit cell angles	90.00, 100.86, 90.00

Refinement procedure

Resolution	34.060 - 2.300
R-factor	0.193
Rwork	0.191
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ZMP
RMSD bond length	0.009
RMSD bond angle	1.321
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Rmerge	0.071
Number of reflections	100467
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	16% PEG4000, 0.2M SODIUM/POTASSIUM PHOSPHATE(PH5.0), pH 8.00, VAPOR DIFFUSION, HANGING DROP, temperature 277K