3AEJ
Reaction intermediate structure of Entamoeba histolytica methionine gamma-lyase 1 tetramer containing Michaelis complex and methionine-pyridoxal-5'-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-12-08 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 98.738, 85.359, 113.664 |
Unit cell angles | 90.00, 101.92, 90.00 |
Refinement procedure
Resolution | 39.040 - 2.590 |
R-factor | 0.212 |
Rwork | 0.209 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3acz |
RMSD bond length | 0.006 |
RMSD bond angle | 0.938 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.680 |
High resolution limit [Å] | 2.590 | 2.590 |
Rmerge | 0.099 | 0.393 |
Number of reflections | 57709 | |
<I/σ(I)> | 12.448 | 3 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 3.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 277 | 1.8M (NH4)2SO4, 0.1M cacodylate buffer, 0.1M Li3(C3H5O(COO)3), 0.1mM pyridozxal 5'-phosphate, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |