3ADI
Structure of Arabidopsis HYL1 and its molecular implications for miRNA processing
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 43 |
| Unit cell lengths | 47.620, 47.620, 115.377 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.720 - 3.200 |
| R-factor | 0.21463 |
| Rwork | 0.204 |
| R-free | 0.31724 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1di2 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.619 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 3783 | |
| <I/σ(I)> | 19.9 | 4.7 |
| Completeness [%] | 97.9 | |
| Redundancy | 7.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG8000, AS, Cacodylate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
