3ADG
Structure of Arabidopsis HYL1 and its molecular implications for miRNA processing
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.388, 37.898, 51.258 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.250 - 1.700 |
| R-factor | 0.20905 |
| Rwork | 0.207 |
| R-free | 0.25356 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1di2 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.376 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 7709 | |
| <I/σ(I)> | 40 | 9 |
| Completeness [%] | 98.4 | 97.4 |
| Redundancy | 5.7 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG400, MgCl2, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
