3AD7
Heterotetrameric Sarcosine Oxidase from Corynebacterium sp. U-96 in complex with methylthio acetate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-26 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 198.949, 198.949, 196.765 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.530 - 2.200 |
| R-factor | 0.17155 |
| Rwork | 0.170 |
| R-free | 0.20455 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x31 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.605 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.620 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.083 | 0.225 |
| Number of reflections | 115870 | |
| <I/σ(I)> | 18.8 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.6 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M TRIS-HCL, 1.9M AMMONIUM SULFATE, 10MM CUSO4, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
