3ACO

Crystal structure of the EFC/F-BAR domain of human PACSIN2/Syndapin II (2.7 A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-01-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97920
Spacegroup name	P 21 21 21
Unit cell lengths	31.607, 84.654, 357.335
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.880 - 2.700
R-factor	0.226
Rwork	0.226
R-free	0.27900
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.000
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SnB
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	27264
<I/σ(I)>	14.3	5.1
Completeness [%]	97.6	90.9
Redundancy	9.5	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	10% PEG 3350, 0.1M calcium chloride, 0.03M glycyl-glycyl-glycine, 0.05 M Tris-HCl, 0.075M NaCl, 0.001M DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K