3ABN
Crystal structure of (Pro-Pro-Gly)4-Hyp-Asp-Gly-(Pro-Pro-Gly)4 at 1.02 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2005-02-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.590, 21.706, 39.151 |
| Unit cell angles | 90.00, 100.17, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.020 |
| Rwork | 0.130 |
| R-free | 0.16300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.026 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.350 | 1.060 |
| High resolution limit [Å] | 1.020 | 1.020 |
| Rmerge | 0.037 | 0.120 |
| Number of reflections | 27010 | |
| <I/σ(I)> | 11.7 | 4.4 |
| Completeness [%] | 99.1 | 99.6 |
| Redundancy | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 15% PEG 4000, 0.05M Ammonium Acetate, 0.05M Sodium Acetate Trihydrate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
