3ABE
Structure of human REV7 in complex with a human REV3 fragment in a tetragonal crystal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 76.517, 76.517, 118.524 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.24242 |
| Rwork | 0.240 |
| R-free | 0.28149 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3abd |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.782 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.057 | 0.314 |
| Number of reflections | 11076 | |
| <I/σ(I)> | 13.2 | 3.1 |
| Completeness [%] | 96.9 | 81.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 8% PEG 20000, 8% PEG 550 MME, 0.8M sodium formate, 0.1M tris-HCl pH 8.5 , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
