3AAE
Crystal structure of Actin capping protein in complex with CARMIL fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-23 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 231.153, 104.358, 186.633 |
Unit cell angles | 90.00, 119.09, 90.00 |
Refinement procedure
Resolution | 49.700 - 3.300 |
R-factor | 0.24472 |
Rwork | 0.243 |
R-free | 0.27092 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3aa0 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.156 |
Data reduction software | DPS |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.700 | 3.420 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.126 | 0.382 |
Number of reflections | 58413 | |
<I/σ(I)> | 16.4 | 5.56 |
Completeness [%] | 99.5 | 99.3 |
Redundancy | 7.6 | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 14% PEG 3350, 180MM TRI-AMMONIUM CITRATE, 100MM MES-NAOH, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |