3AA5

A52F E.coli RNase HI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Spacegroup name	P 21 21 21
Unit cell lengths	42.563, 85.582, 36.277
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.790 - 2.100
R-factor	0.19756
Rwork	0.193
R-free	0.28570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	1.560
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall
Low resolution limit [Å]	42.790
High resolution limit [Å]	2.100
Number of reflections	8637

Crystallization Conditions

crystal ID	method	pH	temperature	details
1