3A95
Crystal structure of hen egg white lysozyme soaked with 100mM RhCl3 at pH3.8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-02-05 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.956, 77.956, 37.305 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.570 - 1.500 |
R-factor | 0.20237 |
Rwork | 0.200 |
R-free | 0.24292 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 193l |
RMSD bond length | 0.008 |
RMSD bond angle | 1.229 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.063 | 0.319 |
Number of reflections | 18981 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 24.4 | 25.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | sodium chloride, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |