3A8S
Crystal structure analysis of the fluorescent protein KillerRed
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-08-04 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 2.29 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 123.464, 123.464, 110.171 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.880 - 2.900 |
R-factor | 0.24737 |
Rwork | 0.245 |
R-free | 0.29818 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.913 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHELXDE |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.950 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.072 | 0.350 |
Number of reflections | 21805 | |
<I/σ(I)> | 74.5 | 13.9 |
Completeness [%] | 97.8 | 96.5 |
Redundancy | 19.7 | 19.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | 293 | 100mM MES pH6.1, 37.5% PEG 400, 5% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |