3A7U
Crystal structure of the bovine lipoyltransferase in its unliganded form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 91.175, 91.175, 232.204 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.440 |
| R-factor | 0.24779 |
| Rwork | 0.242 |
| R-free | 0.31839 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e5a |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.863 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.510 |
| High resolution limit [Å] | 3.440 | 3.450 |
| Rmerge | 0.096 | 0.451 |
| Number of reflections | 8169 | |
| <I/σ(I)> | 44 | 6.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.5 | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |
