3A7R
Crystal structure of E. coli lipoate-protein ligase A in complex with lipoyl-AMP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 102.298, 102.298, 221.252 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.050 |
| R-factor | 0.17701 |
| Rwork | 0.176 |
| R-free | 0.20421 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e5a |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.119 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.122 | 0.472 |
| Number of reflections | 44005 | |
| <I/σ(I)> | 38.1 | 8.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.4 | 20.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | 1.6M Li2SO4, 0.01M MgSO4, 0.05M cacodylate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
