3A7O
The crystal structure of the coiled-coil domain of Saccharomyces cerevisiae Atg16
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2008-07-08 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1.1000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 127.891, 127.891, 77.808 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.080 - 2.500 |
R-factor | 0.273 |
Rwork | 0.272 |
R-free | 0.29900 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.000 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.069 | 0.282 |
Number of reflections | 22847 | |
<I/σ(I)> | 19.4 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 17.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.8M ammonium chloride, 0.1M sodium acetate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.8M ammonium chloride, 0.1M sodium acetate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |