3A75
Crystal structure of glutamate complex of halotolerant γ-glutamyltranspeptidase from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.400, 98.890, 227.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.860 - 1.950 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v36 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.900 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.062 | 0.262 |
| Number of reflections | 76729 | |
| <I/σ(I)> | 18.3 | 4.6 |
| Completeness [%] | 92.5 | 86.2 |
| Redundancy | 5.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 20%(v/v) PEG 4000, 100mM MES pH 7.0, 600mM NaCl, 5% (v/v) Jeffamine M-600, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
