3A6S

Crystal structure of the MutT protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	1999-10-28
Detector	MARRESEARCH
Wavelength(s)	0.7080
Spacegroup name	P 1 21 1
Unit cell lengths	33.902, 71.608, 55.797
Unit cell angles	90.00, 99.03, 90.00

Refinement procedure

Resolution	19.810 - 1.800
R-factor	0.204
Rwork	0.204
R-free	0.23100
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.031	0.094
Number of reflections	24217
<I/σ(I)>	15.8	7.9
Completeness [%]	99.7	99.7
Redundancy	3.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	288	1.4M POTASSIUM SODIUM TARTRATE, 87mM HEPES, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 288K
1	VAPOR DIFFUSION, HANGING DROP	7.5	288	1.4M POTASSIUM SODIUM TARTRATE, 87mM HEPES, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 288K
1	VAPOR DIFFUSION, HANGING DROP	7.5	288	1.4M POTASSIUM SODIUM TARTRATE, 87mM HEPES, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 288K