3A6O

Crystal structure of Thermoactinomyces vulgaris R-47 alpha-amylase 2/acarbose complex

Replaces:  1VFK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	114.599, 119.258, 113.007
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.810 - 2.800
R-factor	0.16402
Rwork	0.157
R-free	0.23106
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.029
RMSD bond angle	2.493
Data reduction software	PROCESS
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.810
High resolution limit [Å]	2.800	2.800
Rmerge	0.084
Number of reflections	35055

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP