3A5U
Promiscuity and specificity in DNA binding to SSB: Insights from the structure of the Mycobacterium smegmatis SSB-ssDNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-03-19 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 112.330, 112.330, 48.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.24035 |
| Rwork | 0.238 |
| R-free | 0.28305 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x3e |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.805 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 8048 | |
| <I/σ(I)> | 15.8 | 2.7 |
| Completeness [%] | 99.6 | 99.1 |
| Redundancy | 6.6 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 100mM Hepes, pH7.5, 30% PEG200, 22% PEG4000, 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Hepes | ||
| 2 | 1 | 1 | PEG200 | ||
| 3 | 1 | 1 | PEG4000 | ||
| 4 | 1 | 1 | glycerol | ||
| 5 | 1 | 2 | Hepes | ||
| 6 | 1 | 2 | PEG200 | ||
| 7 | 1 | 2 | PEG4000 | ||
| 8 | 1 | 2 | glycerol |
