3A5A
Crystal structure of a hemoglobin component V from Propsilocerus akamusi (pH5.6 coordinates)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-04-02 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 65.490, 75.170, 33.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.590 - 1.830 |
| R-factor | 0.19 |
| Rwork | 0.189 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x3k |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.300 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | CNX (2005) |
| Refinement software | CNX (2005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.380 | 1.900 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.047 | 0.150 |
| Number of reflections | 15190 | |
| <I/σ(I)> | 12.5 | |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 13.5 | 13.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | PEG3350, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
