3A58
Crystal structure of Sec3p - Rho1p complex from Saccharomyces cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97924 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 116.000, 116.054, 247.675 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.850 - 2.600 |
R-factor | 0.235 |
Rwork | 0.234 |
R-free | 0.26500 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 52847 | |
<I/σ(I)> | 12.9 | 2.9 |
Completeness [%] | 99.7 | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100mM HEPES-Na (pH6.5), 100mM NaCl, 1.4M ammonium Sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |