3A4V

Crystal structure of pyruvate bound L-Threonine dehydrogenase from Hyperthermophilic Archaeon Thermoplasma volcanium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-02
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	149.803, 45.988, 89.778
Unit cell angles	90.00, 113.37, 90.00

Refinement procedure

Resolution	43.800 - 1.780
Rwork	0.203
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a1n
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.780
Number of reflections	53908

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.4	293	48% MPD, 0.1M Sodium Cacodylate (pH6.4), 5% PEG8000, VAPOR DIFFUSION, SITTING DROP, temperature 293K