3A4S
The crystal structure of the SLD2:Ubc9 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-22 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.250, 49.420, 90.295 |
| Unit cell angles | 103.20, 92.10, 101.13 |
Refinement procedure
| Resolution | 46.366 - 2.700 |
| R-factor | 0.2265 |
| Rwork | 0.222 |
| R-free | 0.26890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries; 1A3S and 3A4R |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.704 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.400 | 2.690 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Rmerge | 0.061 | 0.196 |
| Total number of observations | 645440 | |
| Number of reflections | 13920 | |
| <I/σ(I)> | 18.2 | |
| Completeness [%] | 93.6 | 73.5 |
| Redundancy | 3.4 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 25% polyethylene glycol 1500, 0.1M SPG buffer : SPG buffer was prepared by mixing succinic acid, sodium dihydrogen phosphate, and glycine in the molar ratios 2:7:7, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
