3A4M
Crystal structure of archaeal O-phosphoseryl-tRNA(Sec) kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-15 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.156, 74.524, 64.817 |
Unit cell angles | 90.00, 113.37, 90.00 |
Refinement procedure
Resolution | 46.498 - 1.792 |
R-factor | 0.2114 |
Rwork | 0.209 |
R-free | 0.25250 |
Structure solution method | MAD |
RMSD bond length | 0.023 |
RMSD bond angle | 1.926 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.790 |
Number of reflections | 47081 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 293 | 10mM HEPES-NaOH, 250mM KI and 21% PEG 3350, pH 7.5, VAPOR DIFFUSION, temperature 293K |
1 | VAPOR DIFFUSION | 7.5 | 293 | 10mM HEPES-NaOH, 250mM KI and 21% PEG 3350, pH 7.5, VAPOR DIFFUSION, temperature 293K |