3A4M
Crystal structure of archaeal O-phosphoseryl-tRNA(Sec) kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.156, 74.524, 64.817 |
| Unit cell angles | 90.00, 113.37, 90.00 |
Refinement procedure
| Resolution | 46.498 - 1.792 |
| R-factor | 0.2114 |
| Rwork | 0.209 |
| R-free | 0.25250 |
| Structure solution method | MAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.926 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.790 |
| Number of reflections | 47081 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 10mM HEPES-NaOH, 250mM KI and 21% PEG 3350, pH 7.5, VAPOR DIFFUSION, temperature 293K |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 10mM HEPES-NaOH, 250mM KI and 21% PEG 3350, pH 7.5, VAPOR DIFFUSION, temperature 293K |
