3A3G

Crystal structure of LumP complexed with 6,7-dimethyl-8-(1'-D-ribityl) lumazine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	95
Detector technology	CCD
Collection date	2009-04-28
Detector	ADSC QUANTUM 270
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	46.689, 46.584, 161.587
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.766 - 2.000
R-factor	0.19768
Rwork	0.195
R-free	0.25480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.733
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	44.766
High resolution limit [Å]	2.000
Number of reflections	23305
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	20%(w/v) PEG 4000, 0.2M MgCl2, 100mM Tris-HCl pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K