3A3B
Crystal structure of LumP complexed with flavin mononucleotide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2008-12-05 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.858, 47.087, 160.923 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.220 - 2.000 |
R-factor | 0.20226 |
Rwork | 0.196 |
R-free | 0.25523 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ddy |
RMSD bond length | 0.018 |
RMSD bond angle | 1.904 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 33.220 |
High resolution limit [Å] | 2.000 |
Number of reflections | 22183 |
Completeness [%] | 99.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% (w/v) PEG 4000, 0.2M MgCl2, 100mM Tris-HCl pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |