3A3A

Crystal structure of human selenocystine tRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	90
Detector technology	CCD
Collection date	2008-10-25
Detector	ADSC QUANTUM 315
Wavelength(s)	1.000
Spacegroup name	C 1 2 1
Unit cell lengths	78.949, 51.573, 81.365
Unit cell angles	90.00, 101.64, 90.00

Refinement procedure

Resolution	36.130 - 3.100
R-factor	0.254
Rwork	0.254
R-free	0.31400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2tra 6tna
RMSD bond length	0.005
RMSD bond angle	0.920
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.210
High resolution limit [Å]	3.100	3.100
Rmerge	0.105	0.390
Number of reflections	5833
<I/σ(I)>	18	3.53
Completeness [%]	96.7	85.9
Redundancy	5.4	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.1	293	32% PEG 1500, 0.1M sodium acetate-HCl pH 4.1, 140mM lithium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 293K