3A2P
Structure of 6-aminohexanoate cyclic dimer hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-18 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 62 |
| Unit cell lengths | 130.750, 130.750, 58.230 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.490 - 1.900 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.21400 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.038 | 0.304 |
| Number of reflections | 44300 | |
| <I/σ(I)> | 69.1 | 5.1 |
| Completeness [%] | 98.6 | 90.4 |
| Redundancy | 11 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 283 | 1.0M sodium citrate, 25% (v/v) glycerol, 0.1M imidazole buffer pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 283K |
