3A2G
Crystal Structure of K102C-Myoglobin conjugated with Fluorescein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-12 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 6 |
| Unit cell lengths | 90.409, 90.409, 45.320 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.610 - 1.750 |
| R-factor | 0.17869 |
| Rwork | 0.178 |
| R-free | 0.19746 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2mbw |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.348 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.092 | 0.304 |
| Number of reflections | 21588 | |
| <I/σ(I)> | 30 | 5.4 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 5.5 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | 2.6M ammonium sulfate in 20mM Tris-HCl, pH9.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
