3A23

Crystal Structure of beta-L-Arabinopyranosidase complexed with D-galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	95
Detector technology	CCD
Collection date	2008-11-25
Detector	ADSC QUANTUM 4
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	68.256, 99.081, 181.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.270 - 1.900
R-factor	0.14402
Rwork	0.142
R-free	0.17736
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a21
RMSD bond length	0.009
RMSD bond angle	1.119
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC (5.5.0088)
Refinement software	REFMAC (5.5.0088)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.088	0.286
Number of reflections	97694
<I/σ(I)>	28.4	9.1
Completeness [%]	100.0	100
Redundancy	8.6	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	1.7M ammonium sulfate, 1.7% PEG 400, 15% glycerol, 0.1M HEPES, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K