3A1N
Crystal structure of L-Threonine dehydrogenase from Hyperthermophilic Archaeon Thermoplasma volcanium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 149.759, 45.618, 89.036 |
| Unit cell angles | 90.00, 113.13, 90.00 |
Refinement procedure
| Resolution | 30.340 - 2.070 |
| Rwork | 0.215 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yy7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.240 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.900 | 2.110 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.067 | 0.283 |
| Number of reflections | 33993 | |
| <I/σ(I)> | 36.8 | 7.1 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 5.1 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 293 | 48% MPD, 0.1M Cacodylate (pH6.8), 5% PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
