3A1J

Crystal structure of the human Rad9-Hus1-Rad1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-08
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	77.154, 70.776, 86.661
Unit cell angles	90.00, 99.20, 90.00

Refinement procedure

Resolution	19.900 - 2.500
R-factor	0.209
Rwork	0.209
R-free	0.28200
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	31999
<I/σ(I)>	5.8
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	296	15% PEG 3350, 0.18M KCl, 0.16M guanidine hydrochloride, 0.33M ZWITTERGENT 3-08, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 296K