3A1G
High-Resolution Crystal Structure of RNA polymerase PB1-PB2 subunits from Influenza A Virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-19 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 60.701, 69.987, 61.348 |
| Unit cell angles | 90.00, 97.94, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.24039 |
| Rwork | 0.238 |
| R-free | 0.28967 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ztt |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.845 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.044 | 0.124 |
| Number of reflections | 25865 | |
| <I/σ(I)> | 0 | 25 |
| Completeness [%] | 92.2 | 73 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 80mM Sodium Citrate, 20% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
