3A0R
Crystal structure of histidine kinase ThkA (TM1359) in complex with response regulator protein TrrA (TM1360)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97890, 1.00551, 1.00855, 0.99240 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 110.594, 110.594, 352.508 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.800 |
| R-factor | 0.35094 |
| Rwork | 0.350 |
| R-free | 0.37098 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.203 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.890 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Number of reflections | 11189 | |
| <I/σ(I)> | 22.6 | 1.2 |
| Completeness [%] | 94.9 | 85.1 |
| Redundancy | 7.4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1M Bis-Tris-propane pH 7.0, 1.8M sodium acetate, vapor diffusion, temperature 293K |
