3A0K
Crystal structure of an antiflamatory legume lectin from Cymbosema roseum seeds
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-05 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.43 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.034, 103.074, 122.334 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.120 - 1.800 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cjp |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.699 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.5) |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.920 | 1.890 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.055 | 0.281 |
| Number of reflections | 78960 | |
| <I/σ(I)> | 11.764 | 2.4 |
| Completeness [%] | 98.3 | 94.9 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG3350, Proline, pH8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
