3A0G
Crystal structure analysis of guinea pig oxyhemoglobin at 2.5 angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-12 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 90.370, 84.217, 83.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.890 - 2.500 |
| R-factor | 0.16338 |
| Rwork | 0.159 |
| R-free | 0.21951 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hho |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.388 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.890 | 28.890 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 10470 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 55% PEG 3350 with HPLC grade water, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
