3A0C
Crystal Structure of an anti-HIV mannose-binding lectin from Polygonatum cyrtonema Hua
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Detector technology | CCD |
| Collection date | 2005-12-09 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.308, 48.317, 112.221 |
| Unit cell angles | 90.00, 90.12, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1msa |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.440 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.084 | 0.258 |
| Number of reflections | 28647 | |
| <I/σ(I)> | 4.1 | |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 65% MPD, 5mM EDTA, 0.1M HEPES pH7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
