3A07
Crystal Structure of Actinohivin; Potent anti-HIV Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.07117 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 47.346, 51.633, 175.296 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.300 - 1.190 |
| R-factor | 0.15 |
| Rwork | 0.149 |
| R-free | 0.16300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.223 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.230 |
| High resolution limit [Å] | 1.190 | 1.190 |
| Rmerge | 0.089 | 0.294 |
| Number of reflections | 67499 | |
| <I/σ(I)> | 33.565 | 2.66 |
| Completeness [%] | 97.2 | 84.3 |
| Redundancy | 8.6 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 2-ethoxyehanol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
