39PE

Crystal structure of a septum formation protein Maf from Coccidioides immitis (UMP complex)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2026-09-26
Detector	DECTRIS EIGER2 XE 9M
Wavelength(s)	0.9786
Spacegroup name	P 43 21 2
Unit cell lengths	76.542, 76.542, 176.111
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.580 - 2.400
R-factor	0.2178
Rwork	0.216
R-free	0.24430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.591
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((2.2.1_6174: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.580	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.104	1.978
Rmeas	0.106	2.018
Rpim	0.021	0.397
Total number of observations	552251	55608
Number of reflections	21331	2176
<I/σ(I)>	23.2	1.8
Completeness [%]	100.0
Redundancy	25.9	25.6
CC(1/2)	0.999	0.949

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	Morpheus Fusion D6: 20% (v/v) Ethylene glycol; 10 % (w/v) PEG 8000; 0.04M Imidazole; 0.06M MES monohydrate (acid) pH 6.5; 0.3% w/v NDSB 195, 0.3% w/v NDSB 201, 0.3% w/v NDSB 211, 0.3% w/v NDSB 221, 0.3% w/v NDSB 256 , CoimA.00961.a.A2.PW39528 at 13.2 mg/mL. cocrystallization with 225 mM Uridine 5'-monophosphate (UMP) and 20 mM Magnesium chloride hexahydrate. plate 21394 and G9 drop 2, Puck: PSL-0108, Cryo: direct.