39PC

Crystal structure of a septum formation protein Maf from Coccidioides immitis (sulfate complex)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2026-09-26
Detector	DECTRIS EIGER2 XE 9M
Wavelength(s)	0.9786
Spacegroup name	P 43 21 2
Unit cell lengths	76.869, 76.869, 176.008
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.640 - 2.460
R-factor	0.2221
Rwork	0.221
R-free	0.24680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.806
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((2.2.1_6174: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.640	2.560
High resolution limit [Å]	2.460	2.460
Rmerge	0.115	2.273
Rmeas	0.117	2.317
Rpim	0.023	0.448
Total number of observations	517509	58365
Number of reflections	19993	2202
<I/σ(I)>	21.5	1.8
Completeness [%]	100.0
Redundancy	25.9	26.5
CC(1/2)	1.000	0.879

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	Morpheus Fusion D6: 20% (v/v) PEG 500 MME; 10 % (w/v) PEG 20000; 0.05M Tris (base); 0.05M BICINE pH 8.5; 0.02 M xylitol, 0.02 M D-(−)-fructose, 0.02 M D-sorbitol, 0.02 M myo-inositol, 0.02 M L-rhamnose monohydrate; 0.03 M Lithium sulfate, 0.03 M Sodium sulfate, 0.03 M Potassium sulfate , CoimA.00961.a.A2.PW39528 at 13.2 mg/mL. plate 21394 D6, Puck: PSL-0105, Cryo: direct.