38ZP

SNM1A Nuclease Domain Bound to Phosphorothioate-Modified Single-Stranded DNA in the Presence of Ni and Zn Ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2026-07-22
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 2
Unit cell lengths	90.303, 94.928, 48.241
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.460 - 1.720
R-factor	0.2115
Rwork	0.211
R-free	0.22790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.587
Data reduction software	XDS (VERSION Apr 16, 2026 BUILT=20260616)
Data scaling software	XDS (VERSION Apr 16, 2026 BUILT=20260616)
Phasing software	PHENIX ((2.0_5824: ???))
Refinement software	PHENIX ((2.0_5824: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.464	47.464	1.826
High resolution limit [Å]	1.680	5.019	1.681
Rmerge	0.122	0.081	3.137
Rmeas	0.125	0.083	3.242
Rpim		0.019	0.787
Number of reflections	39403	1970	1970
<I/σ(I)>	13.6	30.1	1.1
Completeness [%]	92.9	100	40.4
Redundancy	13.99
CC(1/2)	0.998	0.993	0.445

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	288	0.1M NaF, 24% PEG3350, 0.1M Bis-Tris prop pH 8.5, 20% ethylene glycol