387D

RNA Pseudoknot with 3D Domain Swapping

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	1995-10-26
Detector	SIEMENS
Spacegroup name	P 43 2 2
Unit cell lengths	61.600, 61.600, 98.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 3.100
R-factor	0.1811
Rwork	0.181
R-free	0.23400
RMSD bond length	0.013
RMSD bond angle	1.610
Data reduction software	XDS
Data scaling software	XDS
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.100
Rmerge	0.051
Number of reflections	3615
Completeness [%]	95.2
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 293.00K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	MGCL2	2	3
2	1	1	SODIUM CACODYLATE	5	6
3	1	1	SODIUM ACETATE	8	9
4	1	2	SODIUM CACODYLATE	11	12
5	1	2	SODIUM ACETATE	14	15