387D
RNA Pseudoknot with 3D Domain Swapping
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-10-26 |
| Detector | SIEMENS |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 61.600, 61.600, 98.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.100 |
| R-factor | 0.1811 |
| Rwork | 0.181 |
| R-free | 0.23400 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.610 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.100 |
| Rmerge | 0.051 |
| Number of reflections | 3615 |
| Completeness [%] | 95.2 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 293.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MGCL2 | 2 | 3 |
| 2 | 1 | 1 | SODIUM CACODYLATE | 5 | 6 |
| 3 | 1 | 1 | SODIUM ACETATE | 8 | 9 |
| 4 | 1 | 2 | SODIUM CACODYLATE | 11 | 12 |
| 5 | 1 | 2 | SODIUM ACETATE | 14 | 15 |
