382D
HYDRATION AND RECOGNITION OF METHYLATED CPG STEPS IN DNA.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 273 |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-03 |
| Detector | SIEMENS |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 41.500, 41.500, 57.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 - 2.200 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| Structure solution method | MOLECULAR REPLECEMENT |
| Starting model (for MR) | STANDARD A-DNA |
| RMSD bond length | 0.006 |
| RMSD bond angle | 26.000 * |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.000 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.056 |
| Number of reflections | 1510 |
| Completeness [%] | 88.9 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | 16 * | pH 6.00 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | SPERMINE | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | dodecamer duplex solution | 0.5 (mM) | |
| 2 | 1 | reservoir | sodium cacodylate | 50 (mM) | |
| 3 | 1 | reservoir | spermine | 0.5 (mM) | |
| 4 | 1 | reservoir | magnesium acetate | 7 (mM) | |
| 5 | 1 | reservoir | 30 (%) |
