37II

Crystal structure of human monoclonal antibody IFA002

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-12-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	85.782, 62.465, 95.730
Unit cell angles	90.00, 91.52, 90.00

Refinement procedure

Resolution	38.750 - 2.150
R-factor	0.2056
Rwork	0.203
R-free	0.25230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	0.879
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.750	2.220
High resolution limit [Å]	2.150	2.150
Number of reflections	27348	2322
<I/σ(I)>	6.6	2.5
Completeness [%]	98.8	96.6
Redundancy	3.5
CC(1/2)	0.972	0.369

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2 M lithium sulfate monohydrate, 0.1 M sodium acetate trihydrate pH 4.5, and 30% w/v PEG 8000