37FZ

OYE1-His6 loop 6 variant - Y304P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-02-26
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920167
Spacegroup name	P 43 21 2
Unit cell lengths	142.269, 142.269, 42.327
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.510 - 2.790
R-factor	0.2776
Rwork	0.272
R-free	0.32680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.197
Data reduction software	XDS
Data scaling software	Aimless (0.7.9)
Phasing software	PHENIX (1.20.1)
Refinement software	PHENIX (1.20.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.510	2.890
High resolution limit [Å]	2.790	2.790
Number of reflections	10380	640
<I/σ(I)>	4.65
Completeness [%]	84.2
Redundancy	7.9
CC(1/2)	0.986	0.384

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.3	277	20 mg/mL protein in 25 mM Tris hydorchloride, 25 mM NaCl, 20% PEG 400, 0.1 M MgCl2, 50 mM HEPES