37ES

OYE1-His6 loop 6 variant - P295A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-02-26
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920167
Spacegroup name	P 21 21 21
Unit cell lengths	138.010, 141.266, 42.073
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.520 - 2.150
R-factor	0.2207
Rwork	0.219
R-free	0.26160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.010
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.520	2.226
High resolution limit [Å]	2.150	2.150
Number of reflections	45824	4505
<I/σ(I)>	5.26
Completeness [%]	99.9
Redundancy	8.5
CC(1/2)	0.990	0.366

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.3	277	20 mg/mL protein in 25 mM Tris hydorchloride, 25 mM NaCl, 20% PEG 400, 0.1 M MgCl2, 50 mM HEPES