379D
THE STRUCTURAL BASIS OF HAMMERHEAD RIBOZYME SELF-CLEAVAGE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-05-09 |
| Detector | CUSTOM-MADE |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 65.100, 65.100, 138.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 3.100 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.28000 * |
| Structure solution method | PHASED FROM ISOMORPHOUS CRYSTAL |
| Starting model (for MR) | HAMMERHEAD RIBOZYME "GROUND STATE" AT PH 6. |
| RMSD bond length | 0.009 |
| RMSD bond angle | 18.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.046 | 0.123 |
| Number of reflections | 6203 | |
| <I/σ(I)> | 12.6 | 5.8 |
| Completeness [%] | 91.6 | 75.1 * |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6 * | pH 8.50, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | LI SULFATE | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | EDTA |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | RNA | 1 (mM) | ||
| 2 | 1 | 2 | 1.8 (M) | ||
| 3 | 1 | 2 | sodium cacodylate | 50 (mM) | |
| 4 | 1 | 2 | 1.5 (mM) |
