377D

5'-R(*CP*GP*UP*AP*CP*DG)-3'

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU R-AXIS II
Temperature [K]	291
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IIC
Spacegroup name	C 2 2 21
Unit cell lengths	29.530, 44.610, 94.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.760
R-factor	0.188
Rwork	0.188
R-free	0.23200
RMSD bond length	0.007 *
RMSD bond angle	1.400 *
Data reduction software	R-AXIS
Data scaling software	R-AXIS
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000 *
High resolution limit [Å]	1.760 *	1.760 *
Rmerge	0.067 *
Number of reflections	4757 *
Completeness [%]	76.0 *	65 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *	291 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	RNA	2 (mM)	single strand concentration
2	1	drop	magnesium acetate	2.5 (mM)
3	1	drop	spermine	0.5 (mM)
4	1	drop	sodium cacodylate	10 (mM)
5	1	drop	DMA	0.2 (mM)
6	1	reservoir	MPD	50 (%)