375D
A NOVEL END-TO-END BINDING OF TWO NETROPSINS TO THE DNA DECAMER D(CCCCCIIIII)2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU |
| Spacegroup name | P 1 |
| Unit cell lengths | 32.560, 32.590, 37.640 |
| Unit cell angles | 86.30, 84.50, 68.58 |
Refinement procedure
| Resolution | 8.000 - 2.400 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.27300 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 18.700 * |
| Data reduction software | MSC |
| Data scaling software | MSC |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.045 |
| Number of reflections | 8546 |
| Completeness [%] | 73.0 |
| Redundancy | 1.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 291.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | NETROPSIN | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | [CO(NH3)6]CL3 | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | NETROPSIN | ||
| 7 | 1 | 2 | NA CACODYLATE | ||
| 8 | 1 | 2 | [CO(NH3)6]CL3 | ||
| 9 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | oligonucleotide | 0.3 (mM) | |
| 2 | 1 | drop | netropsin | 0.3 (mM) | |
| 3 | 1 | drop | sodium cacodylate | 0.4 (mM) | |
| 4 | 1 | drop | cobaltic hexamine chloride | 0.06 (mM) | |
| 5 | 1 | reservoir | MPD | 60 (%) |
